ASADA will continue to present its case against 34 current and former Essendon players charged with doping offences when the AFL Anti-Doping Tribunal reconvenes on January 12.

On Friday, Malcolm Holmes QC, on behalf of the anti-doping authority, continued his submission to the Tribunal, which is being held behind closed doors in the Victorian County Court.

Click here for full coverage of the Essendon anti-doping saga

ASADA is alleging the 34 players and one support person, no longer employed by Essendon, breached the AFL Anti-Doping Code through the use of the banned peptide Thymosin Beta-4.

The anti-doping authority will need to prove its case without first-hand testimony from potential key witnesses Shane Charter and Nima Alavi.

The Supreme Court ruled last Friday that subpoenas could not be issued to biochemist Charter and compound pharmacist Alavi to compel them to give evidence.

On Thursday, Essendon failed in its bid to be represented at the hearing, which is being held in private after a ruling by Tribunal chairman David Jones on December 8.